A CBCT Assessment of Orthodontic Mini-Implant Placement.

Objectives: This study compares the precision of cone beam computed tomography (CBCT) and two-dimensional radiography for mini-implant implantation. Materials and Method: For 30 sites (in 15 patients between the ages of 13 and 26 years), the buccal interradicular region among the 2nd premolar and 1st molar was found to be the best location for mini-implants. Next, two groups of the mini-implant implantation process were created. Mini-implants were positioned at the CBCT data-identified sites in the CBCT group. Mini-implants were inserted in the RVG group by using two-dimensional digital radiography and a specially constructed guide. To assess the precision of the mini-implant implantation, post-placement CBCT images were acquired. The obtained data were statistically analyzed. Result: The two groups showed a statistically considerable variation in the mini-implant placement's departure from the optimal height. Due to the smaller interradicular space and decreased convenience in the posterior mandibular area, two out of 15 mini-implants in the RVG group demonstrated root contact in the mandibular jaw. Conclusion: The two-dimensional intraoral radiograph of the interradicular area provides sufficient information for mini-implant placement even though CBCT accurately visualizes the interradicular space in three dimensions.

An In Vitro Comparative Assessment of Shaping Capacity of a Single-File System Over Multiple-File System in Root Canals.

Objectives: The study's objective was to contrast the two distinct rotary file techniques used to shape mandibular first molars. Materials and Methods: The investigation involved twenty recently removed mandibular molars. A digital single-lens reflex (DSLR) camera was placed in a fixed position, a muffle block was built, and images of each tooth's three cross sections were obtained. The specimens were split into the following two groups at random: Rotary files from WaveOne were used to prepare group A and Mtwo rotary files were used to prepare group B. Results: At the coronal, middle, and apical thirds of the root canals, WaveOne file system demonstrated improved centering ability compared with the Mtwo file system (P 0.05). Conclusion: When compared to the Mtwo file system, WaveOne file system demonstrated improved centering capabilities and needed significantly less time to build the curved canals.

Assessment of Efficiency of Bioactive Glass, Self-Assembling Peptide, and Ozone Remineralising Agents on Artificial Carious Lesion

ABSTRACT Objective: To assess the efficacy of bioactive glass, self-assembling peptide, and ozone-remineralizing agents on the artificial carious lesion. Material and Methods: On the extracted 60 premolar teeth, an artificial carious lesion/demineralization was created. Later, the remineralization of demineralized teeth was done with respective remineralizing agents (Group A: Calcium sodium phosphosilicate (bioactive glass), Group B: Self-assembling peptide, Group C: Ozone remineralizing agents and Group D (Control): De ionized water. The degree of demineralization and remineralization were evaluated using the Vickers Hardness Number. Results: There was a decrease in microhardness from baseline to demineralization in all the groups, and this reduction was found to be statistically considerable. After the remineralization of demineralized samples with respective remineralizing agents, there was an increase in microhardness of 312.38, 276.67, and 254.42 in groups A, B, and C, respectively. In contrast, in Group D, there were no changes. Conclusion: Bioactive glass and self-assembling peptides had higher remineralizing capacities, which can be used to treat early carious lesions.

Evaluation of the Characteristics of Nano Hybrid Composite Resin Modified with Graphene after Thermomechanical Loading Cycle.

To evaluate the characteristics of a thermomechanically loaded composite resin enhanced with graphene nanoparticles. A total of 60 specimens were manufactured with Test group TG1 (20), TG2 (20), and control group CG (20). All samples were subjected to surface roughness using a three-dimensional (3D) Optical profilometer and flexural strength. All specimens were subjected to thermomechanical cyclic loading. Microhardness measurement was performed with a microhardness tester. The average microhardness and flexural strength were higher in test groups TG1 and TG2 and lowest in control group CG. There was an improvement in surface roughness, flexural strength, and microhardness in the test group after the addition of graphene nanoparticles compared to the control group. Conclusion: The addition of graphene nanoparticles to composite resin significantly improved flexural strength and microhardness. The physical and chemical properties of the composite showed marked improvement.

Evaluation of Viability of Periodontal Ligament Cells using Propolis, Coconut Water, Aleo Vera, and Soy Milk: An In vitro Comparative Study.

Objectives: This research was done to assess the periodontal ligament cells viability using propolis, coconut water, aloe vera, and soy milk storage media. Materials and Methods: Periodontal ligament cells were taken from freshly extracted teeth and stored into four groups of storage media: Group A-propolis, Group B-coconut water, Group C-aloe vera, and Group D-soy milk. Later, the cell viability was assessed at 1, 12, and 24 hours. Results: The periodontal ligament cell viability was significantly greater in propolis, followed by coconut water, aloe vera, and soy milk. Conclusion: We found that propolis, coconut water, aloe vera, and soy milk can be used as an effective storage media. These are naturally occurring and easily available storage medium.

An in vitro comparative assessment of manual hand file, rotary protaper ni-ti, erbium:yttrium-aluminum-garnet laser, canal brush, and ultrasound methods for smear layer removal.

Background: Selection of instruments is important to prevent any complications such as ledge formation and instrument breakage. The main drawback linked with instrumentation is smear layer formation. Objectives: This study was done for comparison of manual hand file, rotary ProTaper Ni-Ti, erbium:yttrium-aluminum-garnet (Er:YAG) laser, ultrasound, and CanalBrush (CB) methods for smear layer removal. Methodology: Sixty freshly extracted single-rooted mandibular first premolars extracted for orthodontic treatment purpose were selected and classified into six groups. Group I was negative control in which no final agitation of irrigant was performed. In Group II, solution activation was performed with ProTaper Universal System, in Group III, solution activation was done with canal brush, in Group IV, ultrasound activation was done, in Group V, solution agitation was performed with Er:YAG laser agitation, and in Group VI, canal preparation with hand files was performed. Smear layer score was evaluated after canal preparation with each method. Results: Score 1 was seen in 3 (30%) in Group IV and 6 (60%) in Group V, score 2 was seen in 3 (30%) in Group I, 8 (80%) in Group II, 7 (70%) in Group III, 5 (50%) in Group IV, 3 (30%) in Group V, and 4 (40%) in Group VI. Score 3 was observed in 7 (70%) in Group I, 2 (20%) in Group II, 3 (70%) in Group III, 2 (20%) in Group IV, 1 (10%) in Group V, and 6 (60%) in Group VI. A statistically significant difference was found in smear layer removal score in all groups (P < 0.05) except between Group I versus VI (P > 0.05). Conclusion: Complete removal of the smear layer was not observed in any of the methods used in the study; however, Er: YAG laser was found to be better as compared to other methods.

Résumé Contexte: La sélection des instruments est importante pour éviter toute complication telle que la formation de rebords et la rupture d'instruments. Les Le principal inconvénient lié à l'instrumentation est la formation d'une couche de frottis. Objectifs: Cette étude a été réalisée pour comparer des limes manuelles, méthodes rotatives ProTaper Ni-Ti, laser erbium:yttrium-aluminium-grenat (Er:YAG), ultrasons et CanalBrush (CB) pour l'élimination des frottis. Méthodologie: Soixante premières prémolaires mandibulaires monoracinaires fraîchement extraites à des fins de traitement orthodontique ont été sélectionnées et classés en six groupes. Le groupe I était un témoin négatif dans lequel aucune agitation finale de l'irrigant n'a été effectuée. Dans le groupe II, l'activation de la solution a été réalisée avec le système universel ProTaper, dans le groupe III, l'activation de la solution a été effectuée avec une brosse canalaire, dans le groupe IV, l'activation par ultrasons a été fait, dans le groupe V, l'agitation de la solution a été effectuée avec une agitation au laser Er:YAG, et dans le groupe VI, la préparation du canal avec des limes manuelles a été effectué. Le score de la couche de frottis a été évalué après la préparation du canal avec chaque méthode. Résultats: le score 1 a été observé chez 3 (30 %) dans le groupe IV et 6 (60 %) dans le groupe V, le score 2 a été observé chez 3 (30 %) dans le groupe I, 8 (80 %) dans le groupe II, 7 (70 %) dans le groupe III, 5 (50 %) dans le groupe IV , 3 (30%) dans le groupe V, et 4 (40 %) dans le groupe VI. Le score 3 a été observé chez 7 (70 %) dans le groupe I, 2 (20 %) dans le groupe II, 3 (70 %) dans le groupe III, 2 (20 %) dans Groupe IV, 1 (10 %) dans le groupe V et 6 (60 %) dans le groupe VI. Une différence statistiquement significative a été trouvée dans le score d'élimination des frottis dans tous les groupes (P < 0,05) sauf entre le groupe I et VI (P > 0,05). Conclusion: L'élimination complète de la frottis n'a été observée dans aucun des méthodes utilisées dans l'étude; cependant, le laser Er:YAG s'est avéré meilleur par rapport aux autres méthodes. Mots-clés: Brosse canalaire, erbium : laser yttrium-aluminium-grenat, ProTaper, frottis, ultrasons.

Comparative Evaluation of Apical Sealing Efficacy of Guttaflow Bioseal, Super-Bond Rc Sealer and Adseal Root Canal Sealer.

Objectives: The objectives of this study is to evaluate apical sealing ability of GuttaFlow Bioseal, Super-Bond RC Sealer, and Adseal root canal sealer. Material and Methods: 36 extracted human single rooted permanent teeth, divided into three groups (n = 12) and obturated by lateral condensation technique. Obturation and root canal sealing was done by either Group I: using GuttaFlow Bioseal, Group II: using Super-Bond RC Sealer and Group III: using Adseal sealer, system. Microleakage was assessed by means of dye penetration method. Results: The dye penetration in Group II was more than in Groups I and III in both vertical and horizontal directions, suggesting that GuttaFlow Bioseal and Adseal sealer are effective in reducing the apical leakage compared to Super-Bond RC Sealer. Conclusion: Bioceramic GuttaFlow Bioseal root canal sealers seal are better root canal sealer but they cannot totally eliminate apical leakage.

Comparative Evaluation of Efficacy of Bioactive Glass, Tricalcium Phosphate, and Ozone Remineralizing Agents on Artificial Carious Lesion.

Objectives: Evaluation of efficacy of bioactive glass (BAG), tricalcium phosphate, and ozone remineralizing agents on artificial carious lesion. Materials and Methods: Freshly extracted 48 human premolar teeth were included for this in vitro study. Remineralization of demineralized teeth was done with respective remineralizing agents (Group A - calcium sodium phosphosilicate [BAG], Group B - tricalcium phosphate [Clinpro Tooth Crème], Group C - ozone remineralizing agents, and control group: Group D - deionized water) three times a day for 12 days for 4 min. The degree of demineralization and remineralization was evaluated with Vickers hardness number. Results: There was a decrease in microhardness from baseline to demineralization in all the groups. The difference in microhardness values from demineralization to remineralization in all the three test groups was found to be statistically highly significant. Conclusion: BAG and Tricalcium phosphate had higher remineralizing capacity, which can be used clinically to treat early carious lesions.

Analysis of Fracture Resistance of Endodontically Treated Teeth with Different Restorative Materials: An In vitro Study.

Objectives: The objective of the study is to compare the fracture resistance of endodontic treated tooth restored with GIC, amalgam, and composite cements. Materials and Methods: Forty teeth were divided into 4 groups of 10 teeth each. Group I teeth were restored with silver amalgam. Group II teeth were restored with GIC. Group III teeth were restored with composite and Group IV teeth were control in which no alteration was performed. Testing machine applied force on teeth to detect fracture resistance. Results: The mean fracture resistance was maximum in Group IV (1050.2 N) followed by Group III (956.2 N), Group I (836.4 N), and Group II (766.4 N). The difference was statistically significant (P < 0.05). The mean difference of fracture resistance was significant (P > 0.05). Conclusion: Composite resin possesses highest fractures resistance as compared to GIC and silver amalgam.

Expression of IL-6, TNF-α, and hs-CRP in the serum of patients undergoing single-sitting and multiple-sitting root canal treatment: A comparative study.

Background: In recent times, single-sitting root canal therapy has gained momentum over multiple-sitting root canal therapy due to its superior clinical outcome and less time required for treating the patient. Aim: Thus, the aim of current study was to compare the expression of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and high-sensitivity C-reactive protein (hs-CRP) in the serum of patients undergoing single-sitting and multiple-sitting root canal treatment. Materials and Methods: This cross-sectional experimental study was conducted on 300 subjects who were indicated for root canal treatment. Subjects were categorized into Group I (single visit) and Group II (multiple visits).Clinical data was obtained and serum samples were collected both before and after 1 week of treatment completion. Inclusion criteria were those patients (a) over 18 years of age, (b) without any disease of inflammatory etiology, and (c) who had not previously received endodontic treatment or any related therapeutic treatment. Exclusion criteria were those (a) without a complete clinical history, (b) with greater than one indicated tooth, (c) who did not complete their treatment, and (d) with any periodontal disease. Chi-square and Student's t-test were applied. Results: It was found that in single-sitting root canal treatment, there was a statistically significant reduction in these inflammatory biomarkers, although no difference in clinical efficacy was observed. Conclusion: Single-visit root canal treatment is a better option for treatment of pulpitis compared to multiple-sitting treatment.

Evaluation of Hydroxyapatite Granules, CERAMENT™, and Platelet-rich Fibrin in the Management of Endodontic Apical Surgery.

AIM: The present study compared hydroxyapatite granules, CERAMENT™, and platelet-rich fibrin (PRF) in the management of endodontic apical surgery cases. MATERIALS AND METHODS: The present study was conducted on 126 patients requiring apical surgery of both genders. Patients were treated with hydroxyapatite granules, CERAMENT™, and PRF and were recalled regularly for assessment of pain, mobility, presence or absence of sinus, and healing site. RESULTS: The mean days taken for the disappearance of pain in group I was 51.2 days, in group II was 52.3 days, and in group III was 44.7 days. The difference was significant (p < 0.05). There was a less number of draining sinuses in II and III groups. This was statistically significant (p < 0.05). Significantly less area remained after surgical intervention in groups II and III compared to group I recorded at follow-up period. The difference was significant (p < 0.05). CONCLUSION: Authors found PRF superior in terms of reducing pain, mobility, and sinus and improving the healing site as compared to hydroxyapatite and CERAMENT™. CLINICAL SIGNIFICANCE: Platelet-rich fibrin is considered more superior in terms of reducing pain, mobility, and sinus and improving the healing site, and it can be advised in clinical practice for endodontic management.

Evaluation of Effect of Irrigants with or without Surfactant on Root Canal Transportation by Cone Beam Computed Tomography-An In vitro Study.

INTRODUCTION: Maintenance of original canal anatomy with proper disinfection is our primary goal to achieve during root canal instrumentation. Surfactants are added to irrigating solution to promote deeper penetration into dentinal tubules. AIM: The aim of this study was to evaluate the influence of addition of surfactants to Sodium Hypochlorite (NaOCl) and Ethylenediaminetetraacetic Acid (EDTA) on transportation of root canal. MATERIALS AND METHODS: Fifty human mandibular molars with mesial root curvatures of 10° - 40° were selected and embedded in silicone impression material to simulate mandibular arch form to facilitate imaging process and maintain reproducibility of images. Before instrumentation, root canals were scanned by using Cone Beam Computed Tomography (CBCT) imaging (Carestream, India). The canals were then prepared with the ProTaper Next (PTN) system (Dentsply Maillefer, Ballaigues, Switzerland), using one of the following irrigation regimens during the instrumentation and were divided into five groups based on irrigation regimens followed: G1 (n=10)-irrigation with saline solution(control); G2 (n=10)-irrigation with 2.5% NaOCl; G3 (n=10)-irrigation with 2.5% NaOCl added with surfactant; G4 (n=10)-irrigation with 17% EDTA; G5 (n=10)-irrigation with 17% EDTA added with surfactant. Post-instrumentation scans were obtained with similar parameters and position as pre-instrumentation scans by CBCT imaging. Transportation of the root canals were then analysed at three cross-sectional planes of pre-instrumentation and post-instrumentation images at 2 mm, 5 mm, 8 mm from the apical end of the root. The data was statistically analyzed using Analysis of Variance (ANOVA) and Tukey post hoc test (p<0.05). RESULTS: The mean transportation values were higher in G5. Transportation in G3 and G5 was not significantly different compared to G2 and G4 respectively (p<0.05). CONCLUSION: Instrumentation using irrigating solutions added with surfactant like 1% cetrimide maintained the canal curvature well.

Comparison of Sealer Penetration by Using Different Irrigation Techniques - An In-vitro Study.

INTRODUCTION: The main goal of root canal treatment is to eliminate the microorganisms particularly in the apical third area and to prevent re-infection. In order to achieve these goals the instrumentation must be combined with adequate irrigation. AIM: To compare sealer penetration by using different irrigation techniques i.e., apical negative pressure irrigation, Passive Ultrasonic Irrigation (PUI) and combination of apical negative pressure irrigation and PUI. MATERIALS AND METHODS: A total of 48 single rooted maxillary central incisors were taken. Access cavity was prepared and biomechanical preparation was done. The samples were randomly assigned into three experimental groups based on the final irrigation technique used. Group I: Apical negative pressure (Endovac); Group II: PUI; Group III: Combination of apical negative pressure and PUI. All the samples were obturated using AH plus sealer and the sections were observed under confocal laser scanning microscope to evaluate the percentage and maximum depth of sealer penetration at 1mm, 3mm and 5mm levels. Statistical analysis was done by using two way ANOVA and Tukey's post-hoc test to compare the percentage and maximum depth of sealer penetration. RESULTS: Combination group resulted in better sealer penetration at 1mm and 3mm from the working length than the Endovac and PUI group. However, the Endovac group showed significantly better sealer penetration at 1mm from the working length when compared with PUI. There was no significant difference in sealer penetration at 5mm level between PUI and combination group. CONCLUSION: Combination group was the only group to achieve better sealer penetration at 1mm and 3mm levels from the working length.

Fluoride release and uptake of five dental restoratives from mouthwashes and dentifrices.

BACKGROUND: This study evaluated the fluoride release and uptake of five common dental restoratives mainly glass ionomer formulations, including a conventional glass ionomer, a relatively new caries stabilization glass ionomer and resin-modified glass ionomer (Fuji II, Fuji VII and Fuji II LC); one compomer (F2000); and one fluoride releasing composite resin (tetric ceram). MATERIALS AND METHODS: A total of 12 cylindrical specimens for each of the five materials were prepared following manufacturer's instructions for manipulation and immersed independently in 25 ml of artificial saliva and stored as five groups Group I-V. Each group was further divided into three sub Groups A, B, C. The saliva was changed every day in all the specimens. No treatment was carried out for the specimens in subgroup A. The specimens were immersed in 2% sodium fluoride for 1 min before changing saliva in sub group B and the specimens were treated by brushing with a fluoridated dentifrice for 2 min before changing saliva in sub Group C. The fluoride release was evaluated on the 1(st), 7(th) and 28(th) day using a fluoride ion specific electrode. RESULTS: The results demonstrated that the conventional glass ionomer and the recently introduced caries stabilizing glass ionomer showed similar patterns and quantity of fluoride release, which was significantly higher than the resin-modified glass ionomer, the compomer and the composite resin. The resin-modified glass ionomer showed higher fluoride release than the compomer and the composite resin. All the formulations of glass ionomers showed fluoride uptake from the neutral sodium fluoride and the fluoridated dentifrice, by releasing increased amounts of fluoride after treatment, in comparison with the untreated group. However, the compomer and the composite resin showed no fluoride uptake. CONCLUSION: The fluoride released by the glass ionomer cements (GICs) was found to be highest during the first 24 h and decreased significantly over the 1(st) week with lower levels obtained on the 7(th) and 28(th) day, thus demonstrating the phenomenon of "initial burst." The composite resin and compomer used in this study did not show this phenomenon of the initial burst. The resin-modified GICs released more fluoride than the compomer, and the composite resin.